Hal ini juga bisa menjadi patogen manusia, terkait dengan aspergillosis dari paru-paru dan kadang-kadang menyebabkan infeksi kornea, otomycotic, dan nasoorbital
Banyak strain menghasilkan jumlah yang signifikan aflatoksin , suatu senyawa karsinogenik dan beracun akut. Spora A. flavus yang alergi. A. flavus kadang-kadang menyebabkan kerugian pada tempat penetasan ulat sutera.
A. flavus is the second most common agent of aspergillosis, the first being Aspergillus fumigatus. A. flavus may invade arteries of the lung or brain and cause infarction. Neutropenia predisposes to aspergillus infection. Aspergillus flavus also produces a toxin (aflatoxin) which is one of the aetiological agents for hepatocellular carcinoma. [
Appearance in cultureA. flavus grows as a yellow-green mold in culture. Like other Aspergillus species it produces a distinctive conidiophore composed of a long stalk supporting an inflated vesicle. Conidiogenous cells on the vesicle produce the conidia. Many strains of A. flavus exhibit a greenish fluorescence under UV light that is correlated with levels of aflatoxin production.
 Mold damageA. flavus is particularly common on corn and peanuts, as well as water damaged carpets, and is one of several species of mold known to produce aflatoxin which can cause acute hepatitis, immunosuppression, and hepatocellular carcinoma. The absence of any regulation of screening for the fungus in countries which also have a high prevalence of viral hepatitis highly increases the risk of hepatocellular carcinoma.
To protect tree nuts and corn plants that are affected by A. flavus scientists of the Agricultural Research Service found that treating these plants with the yeast Pichia anomala reduced the growth of A. flavus. The study showed that treating pistachio trees with P. anomala inhibited the growth of A. flavus up to 97% when compared to untreated trees.  The yeast successfully competes with A. flavus for space and nutrients, ultimately limiting the growth of A. flavus.